Recombinant:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: Lot: CT0639 IgGFc Athens Research and Technology Cat# 16-16-090707; RRID:AB_575815 Recombinant mouse Siglec-1 R&D Systems Cat# 5610-SL Recombinant mouse Siglec-2 R&D Systems Cat# 2296-SL Recombinant mouse Siglec-E R&D Systems Cat# 5806-SL Recombinant mouse Siglec-F R&D Systems Cat# 1706-SF Recombinant mouse Siglec-G R&D Systems Cat# 10103-SL Recombinant human Siglec-1 R&D Systems Cat# 5197-SL (Continued on next page) e1 Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human Siglec-2 R&D Systems Cat# 1968-SL Recombinant human Siglec-3 R&D Systems Cat# 1137-SL Recombinant human Siglec-5 R&D Systems Cat# 1072-SL Recombinant human Siglec-6 R&D Systems Cat# 2859-SL Recombinant human Siglec-7 R&D Systems Cat# 1138-SL Recombinant human Siglec-9 R&D Systems Cat# 1139-SL Recombinant human Siglec-10 R&D Systems Cat# 2130-SL Recombinant human Siglec-11 R&D Systems Cat# 3258-SL Human insulin solution Sigma-Aldrich Cat# I9278 Glucose Sigma-Aldrich Cat# G8270 Palmitate Sigma-Aldrich Cat# P9767 BSA, fatty acid free Sigma-Aldrich Cat# A8806 Thioglycollate Sigma-Aldrich Cat# B2551 TRIzol Invitrogen Cat# 15596018 SuperScript First-Strand Synthesis System Thermo Fisher Scientific Cat# 11904018 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4367659 ACK Lysing Buffer Gibco A1049201 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protein A/G-conjugated agarose beads Thermo Fisher Scientific Cat# 20421 Carbo-Free Blocking Solution Vector Laboratories Cat# SP-5040 Neuraminidase A (NanA) This paper N/A Critical commercial assays H&E staining Histoserv, Inc. Cat# ST001A Masson’s trichrome staining Histoserv, Inc. Cat# ST001A BD Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit BD Biosciences Cat# 562246 Mouse TNF Enhanced Sensitivity Flex Set BD Biosciences Cat# 562336 Mouse IL-6 Enhanced Sensitivity Flex Set BD Biosciences Cat# 562236 Total Cholesterol Randox Laboratories Cat# CH200 Triglycerides Randox Laboratories Cat# TR210 HDL Cholesterol Randox Laboratories Cat# CH2652 LDL Cholesterol Randox Laboratories Cat# CH2657 NEFA (Non-Esterified Fatty Acids) Randox Laboratories Cat# FA115 ALT activity assay kit BioVision Cat# K752 AST activity assay kit BioVision Cat# K753 BCA protein assay kit Thermo Fisher Scientific Cat# 23227 Deposited data CD24Fc phase I clinical trial https://clinicaltrials.gov/ NCT02650895 RNA-seq raw data This paper GSE140724 Raw data This paper Data S1 Experimental models: Cell lines Peritoneal macrophages (WT, Cd24 / and Siglece / ) This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6NCr NCI Cat# 556 Mouse: Cd24 / Nielsen et al. (1997) N/A Mouse: Siglece / Flores et al. (2019) N/A Mouse: Siglecg / Ding et al. (2007) N/A (Continued on next page) Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
SYBR Green Assay:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: Lot: CT0639 IgGFc Athens Research and Technology Cat# 16-16-090707; RRID:AB_575815 Recombinant mouse Siglec-1 R&D Systems Cat# 5610-SL Recombinant mouse Siglec-2 R&D Systems Cat# 2296-SL Recombinant mouse Siglec-E R&D Systems Cat# 5806-SL Recombinant mouse Siglec-F R&D Systems Cat# 1706-SF Recombinant mouse Siglec-G R&D Systems Cat# 10103-SL Recombinant human Siglec-1 R&D Systems Cat# 5197-SL (Continued on next page) e1 Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human Siglec-2 R&D Systems Cat# 1968-SL Recombinant human Siglec-3 R&D Systems Cat# 1137-SL Recombinant human Siglec-5 R&D Systems Cat# 1072-SL Recombinant human Siglec-6 R&D Systems Cat# 2859-SL Recombinant human Siglec-7 R&D Systems Cat# 1138-SL Recombinant human Siglec-9 R&D Systems Cat# 1139-SL Recombinant human Siglec-10 R&D Systems Cat# 2130-SL Recombinant human Siglec-11 R&D Systems Cat# 3258-SL Human insulin solution Sigma-Aldrich Cat# I9278 Glucose Sigma-Aldrich Cat# G8270 Palmitate Sigma-Aldrich Cat# P9767 BSA, fatty acid free Sigma-Aldrich Cat# A8806 Thioglycollate Sigma-Aldrich Cat# B2551 TRIzol Invitrogen Cat# 15596018 SuperScript First-Strand Synthesis System Thermo Fisher Scientific Cat# 11904018 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4367659 ACK Lysing Buffer Gibco A1049201 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protein A/G-conjugated agarose beads Thermo Fisher Scientific Cat# 20421 Carbo-Free Blocking Solution Vector Laboratories Cat# SP-5040 Neuraminidase A (NanA) This paper N/A Critical commercial assays H&E staining Histoserv, Inc. Cat# ST001A Masson’s trichrome staining Histoserv, Inc. Cat# ST001A BD Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit BD Biosciences Cat# 562246 Mouse TNF Enhanced Sensitivity Flex Set BD Biosciences Cat# 562336 Mouse IL-6 Enhanced Sensitivity Flex Set BD Biosciences Cat# 562236 Total Cholesterol Randox Laboratories Cat# CH200 Triglycerides Randox Laboratories Cat# TR210 HDL Cholesterol Randox Laboratories Cat# CH2652 LDL Cholesterol Randox Laboratories Cat# CH2657 NEFA (Non-Esterified Fatty Acids) Randox Laboratories Cat# FA115 ALT activity assay kit BioVision Cat# K752 AST activity assay kit BioVision Cat# K753 BCA protein assay kit Thermo Fisher Scientific Cat# 23227 Deposited data CD24Fc phase I clinical trial https://clinicaltrials.gov/ NCT02650895 RNA-seq raw data This paper GSE140724 Raw data This paper Data S1 Experimental models: Cell lines Peritoneal macrophages (WT, Cd24 / and Siglece / ) This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6NCr NCI Cat# 556 Mouse: Cd24 / Nielsen et al. (1997) N/A Mouse: Siglece / Flores et al. (2019) N/A Mouse: Siglecg / Ding et al. (2007) N/A (Continued on next page) Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
Polymerase Chain Reaction:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: Lot: CT0639 IgGFc Athens Research and Technology Cat# 16-16-090707; RRID:AB_575815 Recombinant mouse Siglec-1 R&D Systems Cat# 5610-SL Recombinant mouse Siglec-2 R&D Systems Cat# 2296-SL Recombinant mouse Siglec-E R&D Systems Cat# 5806-SL Recombinant mouse Siglec-F R&D Systems Cat# 1706-SF Recombinant mouse Siglec-G R&D Systems Cat# 10103-SL Recombinant human Siglec-1 R&D Systems Cat# 5197-SL (Continued on next page) e1 Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human Siglec-2 R&D Systems Cat# 1968-SL Recombinant human Siglec-3 R&D Systems Cat# 1137-SL Recombinant human Siglec-5 R&D Systems Cat# 1072-SL Recombinant human Siglec-6 R&D Systems Cat# 2859-SL Recombinant human Siglec-7 R&D Systems Cat# 1138-SL Recombinant human Siglec-9 R&D Systems Cat# 1139-SL Recombinant human Siglec-10 R&D Systems Cat# 2130-SL Recombinant human Siglec-11 R&D Systems Cat# 3258-SL Human insulin solution Sigma-Aldrich Cat# I9278 Glucose Sigma-Aldrich Cat# G8270 Palmitate Sigma-Aldrich Cat# P9767 BSA, fatty acid free Sigma-Aldrich Cat# A8806 Thioglycollate Sigma-Aldrich Cat# B2551 TRIzol Invitrogen Cat# 15596018 SuperScript First-Strand Synthesis System Thermo Fisher Scientific Cat# 11904018 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4367659 ACK Lysing Buffer Gibco A1049201 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protein A/G-conjugated agarose beads Thermo Fisher Scientific Cat# 20421 Carbo-Free Blocking Solution Vector Laboratories Cat# SP-5040 Neuraminidase A (NanA) This paper N/A Critical commercial assays H&E staining Histoserv, Inc. Cat# ST001A Masson’s trichrome staining Histoserv, Inc. Cat# ST001A BD Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit BD Biosciences Cat# 562246 Mouse TNF Enhanced Sensitivity Flex Set BD Biosciences Cat# 562336 Mouse IL-6 Enhanced Sensitivity Flex Set BD Biosciences Cat# 562236 Total Cholesterol Randox Laboratories Cat# CH200 Triglycerides Randox Laboratories Cat# TR210 HDL Cholesterol Randox Laboratories Cat# CH2652 LDL Cholesterol Randox Laboratories Cat# CH2657 NEFA (Non-Esterified Fatty Acids) Randox Laboratories Cat# FA115 ALT activity assay kit BioVision Cat# K752 AST activity assay kit BioVision Cat# K753 BCA protein assay kit Thermo Fisher Scientific Cat# 23227 Deposited data CD24Fc phase I clinical trial https://clinicaltrials.gov/ NCT02650895 RNA-seq raw data This paper GSE140724 Raw data This paper Data S1 Experimental models: Cell lines Peritoneal macrophages (WT, Cd24 / and Siglece / ) This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6NCr NCI Cat# 556 Mouse: Cd24 / Nielsen et al. (1997) N/A Mouse: Siglece / Flores et al. (2019) N/A Mouse: Siglecg / Ding et al. (2007) N/A (Continued on next page) Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
Blocking Assay:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: Lot: CT0639 IgGFc Athens Research and Technology Cat# 16-16-090707; RRID:AB_575815 Recombinant mouse Siglec-1 R&D Systems Cat# 5610-SL Recombinant mouse Siglec-2 R&D Systems Cat# 2296-SL Recombinant mouse Siglec-E R&D Systems Cat# 5806-SL Recombinant mouse Siglec-F R&D Systems Cat# 1706-SF Recombinant mouse Siglec-G R&D Systems Cat# 10103-SL Recombinant human Siglec-1 R&D Systems Cat# 5197-SL (Continued on next page) e1 Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human Siglec-2 R&D Systems Cat# 1968-SL Recombinant human Siglec-3 R&D Systems Cat# 1137-SL Recombinant human Siglec-5 R&D Systems Cat# 1072-SL Recombinant human Siglec-6 R&D Systems Cat# 2859-SL Recombinant human Siglec-7 R&D Systems Cat# 1138-SL Recombinant human Siglec-9 R&D Systems Cat# 1139-SL Recombinant human Siglec-10 R&D Systems Cat# 2130-SL Recombinant human Siglec-11 R&D Systems Cat# 3258-SL Human insulin solution Sigma-Aldrich Cat# I9278 Glucose Sigma-Aldrich Cat# G8270 Palmitate Sigma-Aldrich Cat# P9767 BSA, fatty acid free Sigma-Aldrich Cat# A8806 Thioglycollate Sigma-Aldrich Cat# B2551 TRIzol Invitrogen Cat# 15596018 SuperScript First-Strand Synthesis System Thermo Fisher Scientific Cat# 11904018 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4367659 ACK Lysing Buffer Gibco A1049201 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protein A/G-conjugated agarose beads Thermo Fisher Scientific Cat# 20421 Carbo-Free Blocking Solution Vector Laboratories Cat# SP-5040 Neuraminidase A (NanA) This paper N/A Critical commercial assays H&E staining Histoserv, Inc. Cat# ST001A Masson’s trichrome staining Histoserv, Inc. Cat# ST001A BD Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit BD Biosciences Cat# 562246 Mouse TNF Enhanced Sensitivity Flex Set BD Biosciences Cat# 562336 Mouse IL-6 Enhanced Sensitivity Flex Set BD Biosciences Cat# 562236 Total Cholesterol Randox Laboratories Cat# CH200 Triglycerides Randox Laboratories Cat# TR210 HDL Cholesterol Randox Laboratories Cat# CH2652 LDL Cholesterol Randox Laboratories Cat# CH2657 NEFA (Non-Esterified Fatty Acids) Randox Laboratories Cat# FA115 ALT activity assay kit BioVision Cat# K752 AST activity assay kit BioVision Cat# K753 BCA protein assay kit Thermo Fisher Scientific Cat# 23227 Deposited data CD24Fc phase I clinical trial https://clinicaltrials.gov/ NCT02650895 RNA-seq raw data This paper GSE140724 Raw data This paper Data S1 Experimental models: Cell lines Peritoneal macrophages (WT, Cd24 / and Siglece / ) This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6NCr NCI Cat# 556 Mouse: Cd24 / Nielsen et al. (1997) N/A Mouse: Siglece / Flores et al. (2019) N/A Mouse: Siglecg / Ding et al. (2007) N/A (Continued on next page) Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
Staining:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: Lot: CT0639 IgGFc Athens Research and Technology Cat# 16-16-090707; RRID:AB_575815 Recombinant mouse Siglec-1 R&D Systems Cat# 5610-SL Recombinant mouse Siglec-2 R&D Systems Cat# 2296-SL Recombinant mouse Siglec-E R&D Systems Cat# 5806-SL Recombinant mouse Siglec-F R&D Systems Cat# 1706-SF Recombinant mouse Siglec-G R&D Systems Cat# 10103-SL Recombinant human Siglec-1 R&D Systems Cat# 5197-SL (Continued on next page) e1 Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human Siglec-2 R&D Systems Cat# 1968-SL Recombinant human Siglec-3 R&D Systems Cat# 1137-SL Recombinant human Siglec-5 R&D Systems Cat# 1072-SL Recombinant human Siglec-6 R&D Systems Cat# 2859-SL Recombinant human Siglec-7 R&D Systems Cat# 1138-SL Recombinant human Siglec-9 R&D Systems Cat# 1139-SL Recombinant human Siglec-10 R&D Systems Cat# 2130-SL Recombinant human Siglec-11 R&D Systems Cat# 3258-SL Human insulin solution Sigma-Aldrich Cat# I9278 Glucose Sigma-Aldrich Cat# G8270 Palmitate Sigma-Aldrich Cat# P9767 BSA, fatty acid free Sigma-Aldrich Cat# A8806 Thioglycollate Sigma-Aldrich Cat# B2551 TRIzol Invitrogen Cat# 15596018 SuperScript First-Strand Synthesis System Thermo Fisher Scientific Cat# 11904018 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4367659 ACK Lysing Buffer Gibco A1049201 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protein A/G-conjugated agarose beads Thermo Fisher Scientific Cat# 20421 Carbo-Free Blocking Solution Vector Laboratories Cat# SP-5040 Neuraminidase A (NanA) This paper N/A Critical commercial assays H&E staining Histoserv, Inc. Cat# ST001A Masson’s trichrome staining Histoserv, Inc. Cat# ST001A BD Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit BD Biosciences Cat# 562246 Mouse TNF Enhanced Sensitivity Flex Set BD Biosciences Cat# 562336 Mouse IL-6 Enhanced Sensitivity Flex Set BD Biosciences Cat# 562236 Total Cholesterol Randox Laboratories Cat# CH200 Triglycerides Randox Laboratories Cat# TR210 HDL Cholesterol Randox Laboratories Cat# CH2652 LDL Cholesterol Randox Laboratories Cat# CH2657 NEFA (Non-Esterified Fatty Acids) Randox Laboratories Cat# FA115 ALT activity assay kit BioVision Cat# K752 AST activity assay kit BioVision Cat# K753 BCA protein assay kit Thermo Fisher Scientific Cat# 23227 Deposited data CD24Fc phase I clinical trial https://clinicaltrials.gov/ NCT02650895 RNA-seq raw data This paper GSE140724 Raw data This paper Data S1 Experimental models: Cell lines Peritoneal macrophages (WT, Cd24 / and Siglece / ) This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6NCr NCI Cat# 556 Mouse: Cd24 / Nielsen et al. (1997) N/A Mouse: Siglece / Flores et al. (2019) N/A Mouse: Siglecg / Ding et al. (2007) N/A (Continued on next page) Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
Crocin Bleaching Assay:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: Lot: CT0639 IgGFc Athens Research and Technology Cat# 16-16-090707; RRID:AB_575815 Recombinant mouse Siglec-1 R&D Systems Cat# 5610-SL Recombinant mouse Siglec-2 R&D Systems Cat# 2296-SL Recombinant mouse Siglec-E R&D Systems Cat# 5806-SL Recombinant mouse Siglec-F R&D Systems Cat# 1706-SF Recombinant mouse Siglec-G R&D Systems Cat# 10103-SL Recombinant human Siglec-1 R&D Systems Cat# 5197-SL (Continued on next page) e1 Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human Siglec-2 R&D Systems Cat# 1968-SL Recombinant human Siglec-3 R&D Systems Cat# 1137-SL Recombinant human Siglec-5 R&D Systems Cat# 1072-SL Recombinant human Siglec-6 R&D Systems Cat# 2859-SL Recombinant human Siglec-7 R&D Systems Cat# 1138-SL Recombinant human Siglec-9 R&D Systems Cat# 1139-SL Recombinant human Siglec-10 R&D Systems Cat# 2130-SL Recombinant human Siglec-11 R&D Systems Cat# 3258-SL Human insulin solution Sigma-Aldrich Cat# I9278 Glucose Sigma-Aldrich Cat# G8270 Palmitate Sigma-Aldrich Cat# P9767 BSA, fatty acid free Sigma-Aldrich Cat# A8806 Thioglycollate Sigma-Aldrich Cat# B2551 TRIzol Invitrogen Cat# 15596018 SuperScript First-Strand Synthesis System Thermo Fisher Scientific Cat# 11904018 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4367659 ACK Lysing Buffer Gibco A1049201 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protein A/G-conjugated agarose beads Thermo Fisher Scientific Cat# 20421 Carbo-Free Blocking Solution Vector Laboratories Cat# SP-5040 Neuraminidase A (NanA) This paper N/A Critical commercial assays H&E staining Histoserv, Inc. Cat# ST001A Masson’s trichrome staining Histoserv, Inc. Cat# ST001A BD Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit BD Biosciences Cat# 562246 Mouse TNF Enhanced Sensitivity Flex Set BD Biosciences Cat# 562336 Mouse IL-6 Enhanced Sensitivity Flex Set BD Biosciences Cat# 562236 Total Cholesterol Randox Laboratories Cat# CH200 Triglycerides Randox Laboratories Cat# TR210 HDL Cholesterol Randox Laboratories Cat# CH2652 LDL Cholesterol Randox Laboratories Cat# CH2657 NEFA (Non-Esterified Fatty Acids) Randox Laboratories Cat# FA115 ALT activity assay kit BioVision Cat# K752 AST activity assay kit BioVision Cat# K753 BCA protein assay kit Thermo Fisher Scientific Cat# 23227 Deposited data CD24Fc phase I clinical trial https://clinicaltrials.gov/ NCT02650895 RNA-seq raw data This paper GSE140724 Raw data This paper Data S1 Experimental models: Cell lines Peritoneal macrophages (WT, Cd24 / and Siglece / ) This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6NCr NCI Cat# 556 Mouse: Cd24 / Nielsen et al. (1997) N/A Mouse: Siglece / Flores et al. (2019) N/A Mouse: Siglecg / Ding et al. (2007) N/A (Continued on next page) Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
Activity Assay:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: Lot: CT0639 IgGFc Athens Research and Technology Cat# 16-16-090707; RRID:AB_575815 Recombinant mouse Siglec-1 R&D Systems Cat# 5610-SL Recombinant mouse Siglec-2 R&D Systems Cat# 2296-SL Recombinant mouse Siglec-E R&D Systems Cat# 5806-SL Recombinant mouse Siglec-F R&D Systems Cat# 1706-SF Recombinant mouse Siglec-G R&D Systems Cat# 10103-SL Recombinant human Siglec-1 R&D Systems Cat# 5197-SL (Continued on next page) e1 Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human Siglec-2 R&D Systems Cat# 1968-SL Recombinant human Siglec-3 R&D Systems Cat# 1137-SL Recombinant human Siglec-5 R&D Systems Cat# 1072-SL Recombinant human Siglec-6 R&D Systems Cat# 2859-SL Recombinant human Siglec-7 R&D Systems Cat# 1138-SL Recombinant human Siglec-9 R&D Systems Cat# 1139-SL Recombinant human Siglec-10 R&D Systems Cat# 2130-SL Recombinant human Siglec-11 R&D Systems Cat# 3258-SL Human insulin solution Sigma-Aldrich Cat# I9278 Glucose Sigma-Aldrich Cat# G8270 Palmitate Sigma-Aldrich Cat# P9767 BSA, fatty acid free Sigma-Aldrich Cat# A8806 Thioglycollate Sigma-Aldrich Cat# B2551 TRIzol Invitrogen Cat# 15596018 SuperScript First-Strand Synthesis System Thermo Fisher Scientific Cat# 11904018 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4367659 ACK Lysing Buffer Gibco A1049201 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protein A/G-conjugated agarose beads Thermo Fisher Scientific Cat# 20421 Carbo-Free Blocking Solution Vector Laboratories Cat# SP-5040 Neuraminidase A (NanA) This paper N/A Critical commercial assays H&E staining Histoserv, Inc. Cat# ST001A Masson’s trichrome staining Histoserv, Inc. Cat# ST001A BD Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit BD Biosciences Cat# 562246 Mouse TNF Enhanced Sensitivity Flex Set BD Biosciences Cat# 562336 Mouse IL-6 Enhanced Sensitivity Flex Set BD Biosciences Cat# 562236 Total Cholesterol Randox Laboratories Cat# CH200 Triglycerides Randox Laboratories Cat# TR210 HDL Cholesterol Randox Laboratories Cat# CH2652 LDL Cholesterol Randox Laboratories Cat# CH2657 NEFA (Non-Esterified Fatty Acids) Randox Laboratories Cat# FA115 ALT activity assay kit BioVision Cat# K752 AST activity assay kit BioVision Cat# K753 BCA protein assay kit Thermo Fisher Scientific Cat# 23227 Deposited data CD24Fc phase I clinical trial https://clinicaltrials.gov/ NCT02650895 RNA-seq raw data This paper GSE140724 Raw data This paper Data S1 Experimental models: Cell lines Peritoneal macrophages (WT, Cd24 / and Siglece / ) This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6NCr NCI Cat# 556 Mouse: Cd24 / Nielsen et al. (1997) N/A Mouse: Siglece / Flores et al. (2019) N/A Mouse: Siglecg / Ding et al. (2007) N/A (Continued on next page) Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
AST Assay:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: Lot: CT0639 IgGFc Athens Research and Technology Cat# 16-16-090707; RRID:AB_575815 Recombinant mouse Siglec-1 R&D Systems Cat# 5610-SL Recombinant mouse Siglec-2 R&D Systems Cat# 2296-SL Recombinant mouse Siglec-E R&D Systems Cat# 5806-SL Recombinant mouse Siglec-F R&D Systems Cat# 1706-SF Recombinant mouse Siglec-G R&D Systems Cat# 10103-SL Recombinant human Siglec-1 R&D Systems Cat# 5197-SL (Continued on next page) e1 Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human Siglec-2 R&D Systems Cat# 1968-SL Recombinant human Siglec-3 R&D Systems Cat# 1137-SL Recombinant human Siglec-5 R&D Systems Cat# 1072-SL Recombinant human Siglec-6 R&D Systems Cat# 2859-SL Recombinant human Siglec-7 R&D Systems Cat# 1138-SL Recombinant human Siglec-9 R&D Systems Cat# 1139-SL Recombinant human Siglec-10 R&D Systems Cat# 2130-SL Recombinant human Siglec-11 R&D Systems Cat# 3258-SL Human insulin solution Sigma-Aldrich Cat# I9278 Glucose Sigma-Aldrich Cat# G8270 Palmitate Sigma-Aldrich Cat# P9767 BSA, fatty acid free Sigma-Aldrich Cat# A8806 Thioglycollate Sigma-Aldrich Cat# B2551 TRIzol Invitrogen Cat# 15596018 SuperScript First-Strand Synthesis System Thermo Fisher Scientific Cat# 11904018 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4367659 ACK Lysing Buffer Gibco A1049201 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protein A/G-conjugated agarose beads Thermo Fisher Scientific Cat# 20421 Carbo-Free Blocking Solution Vector Laboratories Cat# SP-5040 Neuraminidase A (NanA) This paper N/A Critical commercial assays H&E staining Histoserv, Inc. Cat# ST001A Masson’s trichrome staining Histoserv, Inc. Cat# ST001A BD Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit BD Biosciences Cat# 562246 Mouse TNF Enhanced Sensitivity Flex Set BD Biosciences Cat# 562336 Mouse IL-6 Enhanced Sensitivity Flex Set BD Biosciences Cat# 562236 Total Cholesterol Randox Laboratories Cat# CH200 Triglycerides Randox Laboratories Cat# TR210 HDL Cholesterol Randox Laboratories Cat# CH2652 LDL Cholesterol Randox Laboratories Cat# CH2657 NEFA (Non-Esterified Fatty Acids) Randox Laboratories Cat# FA115 ALT activity assay kit BioVision Cat# K752 AST activity assay kit BioVision Cat# K753 BCA protein assay kit Thermo Fisher Scientific Cat# 23227 Deposited data CD24Fc phase I clinical trial https://clinicaltrials.gov/ NCT02650895 RNA-seq raw data This paper GSE140724 Raw data This paper Data S1 Experimental models: Cell lines Peritoneal macrophages (WT, Cd24 / and Siglece / ) This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6NCr NCI Cat# 556 Mouse: Cd24 / Nielsen et al. (1997) N/A Mouse: Siglece / Flores et al. (2019) N/A Mouse: Siglecg / Ding et al. (2007) N/A (Continued on next page) Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
Bicinchoninic Acid Protein Assay:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: Lot: CT0639 IgGFc Athens Research and Technology Cat# 16-16-090707; RRID:AB_575815 Recombinant mouse Siglec-1 R&D Systems Cat# 5610-SL Recombinant mouse Siglec-2 R&D Systems Cat# 2296-SL Recombinant mouse Siglec-E R&D Systems Cat# 5806-SL Recombinant mouse Siglec-F R&D Systems Cat# 1706-SF Recombinant mouse Siglec-G R&D Systems Cat# 10103-SL Recombinant human Siglec-1 R&D Systems Cat# 5197-SL (Continued on next page) e1 Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human Siglec-2 R&D Systems Cat# 1968-SL Recombinant human Siglec-3 R&D Systems Cat# 1137-SL Recombinant human Siglec-5 R&D Systems Cat# 1072-SL Recombinant human Siglec-6 R&D Systems Cat# 2859-SL Recombinant human Siglec-7 R&D Systems Cat# 1138-SL Recombinant human Siglec-9 R&D Systems Cat# 1139-SL Recombinant human Siglec-10 R&D Systems Cat# 2130-SL Recombinant human Siglec-11 R&D Systems Cat# 3258-SL Human insulin solution Sigma-Aldrich Cat# I9278 Glucose Sigma-Aldrich Cat# G8270 Palmitate Sigma-Aldrich Cat# P9767 BSA, fatty acid free Sigma-Aldrich Cat# A8806 Thioglycollate Sigma-Aldrich Cat# B2551 TRIzol Invitrogen Cat# 15596018 SuperScript First-Strand Synthesis System Thermo Fisher Scientific Cat# 11904018 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4367659 ACK Lysing Buffer Gibco A1049201 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protein A/G-conjugated agarose beads Thermo Fisher Scientific Cat# 20421 Carbo-Free Blocking Solution Vector Laboratories Cat# SP-5040 Neuraminidase A (NanA) This paper N/A Critical commercial assays H&E staining Histoserv, Inc. Cat# ST001A Masson’s trichrome staining Histoserv, Inc. Cat# ST001A BD Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit BD Biosciences Cat# 562246 Mouse TNF Enhanced Sensitivity Flex Set BD Biosciences Cat# 562336 Mouse IL-6 Enhanced Sensitivity Flex Set BD Biosciences Cat# 562236 Total Cholesterol Randox Laboratories Cat# CH200 Triglycerides Randox Laboratories Cat# TR210 HDL Cholesterol Randox Laboratories Cat# CH2652 LDL Cholesterol Randox Laboratories Cat# CH2657 NEFA (Non-Esterified Fatty Acids) Randox Laboratories Cat# FA115 ALT activity assay kit BioVision Cat# K752 AST activity assay kit BioVision Cat# K753 BCA protein assay kit Thermo Fisher Scientific Cat# 23227 Deposited data CD24Fc phase I clinical trial https://clinicaltrials.gov/ NCT02650895 RNA-seq raw data This paper GSE140724 Raw data This paper Data S1 Experimental models: Cell lines Peritoneal macrophages (WT, Cd24 / and Siglece / ) This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6NCr NCI Cat# 556 Mouse: Cd24 / Nielsen et al. (1997) N/A Mouse: Siglece / Flores et al. (2019) N/A Mouse: Siglecg / Ding et al. (2007) N/A (Continued on next page) Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
RNA Sequencing:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: Lot: CT0639 IgGFc Athens Research and Technology Cat# 16-16-090707; RRID:AB_575815 Recombinant mouse Siglec-1 R&D Systems Cat# 5610-SL Recombinant mouse Siglec-2 R&D Systems Cat# 2296-SL Recombinant mouse Siglec-E R&D Systems Cat# 5806-SL Recombinant mouse Siglec-F R&D Systems Cat# 1706-SF Recombinant mouse Siglec-G R&D Systems Cat# 10103-SL Recombinant human Siglec-1 R&D Systems Cat# 5197-SL (Continued on next page) e1 Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human Siglec-2 R&D Systems Cat# 1968-SL Recombinant human Siglec-3 R&D Systems Cat# 1137-SL Recombinant human Siglec-5 R&D Systems Cat# 1072-SL Recombinant human Siglec-6 R&D Systems Cat# 2859-SL Recombinant human Siglec-7 R&D Systems Cat# 1138-SL Recombinant human Siglec-9 R&D Systems Cat# 1139-SL Recombinant human Siglec-10 R&D Systems Cat# 2130-SL Recombinant human Siglec-11 R&D Systems Cat# 3258-SL Human insulin solution Sigma-Aldrich Cat# I9278 Glucose Sigma-Aldrich Cat# G8270 Palmitate Sigma-Aldrich Cat# P9767 BSA, fatty acid free Sigma-Aldrich Cat# A8806 Thioglycollate Sigma-Aldrich Cat# B2551 TRIzol Invitrogen Cat# 15596018 SuperScript First-Strand Synthesis System Thermo Fisher Scientific Cat# 11904018 Power SYBR Green PCR Master Mix Applied Biosystems Cat# 4367659 ACK Lysing Buffer Gibco A1049201 RIPA buffer Thermo Fisher Scientific Cat# 89900 Protein A/G-conjugated agarose beads Thermo Fisher Scientific Cat# 20421 Carbo-Free Blocking Solution Vector Laboratories Cat# SP-5040 Neuraminidase A (NanA) This paper N/A Critical commercial assays H&E staining Histoserv, Inc. Cat# ST001A Masson’s trichrome staining Histoserv, Inc. Cat# ST001A BD Cytometric Bead Array (CBA) Mouse Enhanced Sensitivity Master Buffer Kit BD Biosciences Cat# 562246 Mouse TNF Enhanced Sensitivity Flex Set BD Biosciences Cat# 562336 Mouse IL-6 Enhanced Sensitivity Flex Set BD Biosciences Cat# 562236 Total Cholesterol Randox Laboratories Cat# CH200 Triglycerides Randox Laboratories Cat# TR210 HDL Cholesterol Randox Laboratories Cat# CH2652 LDL Cholesterol Randox Laboratories Cat# CH2657 NEFA (Non-Esterified Fatty Acids) Randox Laboratories Cat# FA115 ALT activity assay kit BioVision Cat# K752 AST activity assay kit BioVision Cat# K753 BCA protein assay kit Thermo Fisher Scientific Cat# 23227 Deposited data CD24Fc phase I clinical trial https://clinicaltrials.gov/ NCT02650895 RNA-seq raw data This paper GSE140724 Raw data This paper Data S1 Experimental models: Cell lines Peritoneal macrophages (WT, Cd24 / and Siglece / ) This paper N/A Experimental models: Organisms/strains Mouse: C57BL/6NCr NCI Cat# 556 Mouse: Cd24 / Nielsen et al. (1997) N/A Mouse: Siglece / Flores et al. (2019) N/A Mouse: Siglecg / Ding et al. (2007) N/A (Continued on next page) Cell Metabolism 34, 1088–1103.e1–e6, August 2, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
other:Article Title: CD24-Siglec axis is an innate immune checkpoint against metaflammation and metabolic disorder.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Siglec2 / This paper N/A Mouse: Siglec3 / This paper N/A Mouse: Siglech / This paper N/A Mouse: Siglec2 / /h / This paper N/A Mouse: Siglec3 / /f / This paper N/A Mouse: Siglecf / /g / This paper N/A Mouse: Siglecg / /h / This paper N/A Mouse: Siglecf / /g / /h / This paper N/A Oligonucleotides Primers for qPCR, see Table S2 This paper N/A Software and algorithms Graphpad Prism 8 Graphpad https://www.graphpad.com Flowjo_V10 BD Biosciences https://www.flowjo.com Image-Pro Plus 6 Media Cybernetics http://www.mediacy.com/imageproplus Other High-fat diet Research Diets Cat# D12492 Choline-deficient HFD Research Diets Cat# A06071302
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